[KO Validated] HIF1AN/FIH1 Rabbit pAb - A5466

[KO Validated] HIF1AN/FIH1 Rabbit pAb

Sizes: 20μL, 100μL

Catalogue Numbers: A5466-20, A5466-100

Citations, Manuals and MSDS Available upon request.

Background: Enables several functions, including 2-oxoglutarate-dependent dioxygenase activity; NF-kappaB binding activity; and transition metal ion binding activity. Involved in several processes, including negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter in response to hypoxia; and protein hydroxylation. Located in cytosol; nucleoplasm; and perinuclear region of cytoplasm. Colocalizes with nucleus.

Category: Polyclonal Antibodies

Applications: WB, IHC-P, IF/ICC, IP, ELISA

Cross-reactivity: Human, Mouse, Rat

Protein Weight: 41kDa

Observed Molecular Weight: 40kDa

Immunogen: Recombinant protein (or fragment) .

Species: Human

GeneID: 55662

SWISS: Q9NWT6

Alternate Names: FIH1; H1

Source: Rabbit

Isotype: IgG

Purity: Affinity purification

Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis),  pH 7.3.

Recommended Dilutions: WB, 1:500 - 1:2000 IHC-P, 1:50 - 1:200 IF/ICC, 1:50 - 1:200 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Research Areas: Epigenetics Nuclear Signaling, Cancer, Invasion and Metastasis, Cardiovascular, Hypoxia.

NCBI Alias: HIF1AN

Research Use Only