MAP3K4 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A16918-20, A16918-100
Citations, Manuals and MSDS Available upon request.
Background: The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, ELISA
Cross-reactivity: Human, Rat
Protein Weight: 182kDa
Observed Molecular Weight: 170kDa
Immunogen: Synthetic peptide
Species: Human
GeneID: 4216
SWISS: Q9Y6R4
Alternate Names: MTK1; MEKK4; MEKK 4; MAPKKK4; PRO0412; MAP3K4
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 IHC-P, 1:100 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Signal Transduction, Kinase, MAPK-JNK Signaling Pathway, Cell Biology Developmental Biology, Cytoskeleton, Actins, Immunology Inflammation, B Cell Receptor Signaling Pathway, Neuroscience.
NCBI Alias: MAP3K4
Research Use Only