mNeonGreen Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A24858-20, A24858-100
Citations, Manuals and MSDS Available upon request.
Background: Mutations were performed on LanYFP, and mNeonGreen (GenBank login number KC295282) was identified. In addition to the additional EGFP type terminal 9, a total of 21 mutations were found relative to the tetramer LanYFP (F15I, R25Q, A45D, Q56H, F67Y, K79V, S100V, F115A, I118K, V140R, T141S, M143K, L144T, D156K, T158S, S163N, Q168R, V171A, N174T, I185Y, F192Y). It is a yellow green fluorescent protein derived from amphioxus. As a fusion protein, mNeonGreen is a very useful tool for reporting gene expression, tracking cell lines, and determining subcellular protein localization.On the protein sequence level, only 20-25% of the mNeonGreen sequences are consistent with ordinary GFP derivative sequences; MNeonGreen has a maximum excitation value at 506 nm and a maximum emission value at 517 nm (Shaner et al., 2013). MNeonGreen is compatible with most GFP filter sets. Obviously, when using a GFP filter, it is three times brighter than GFP; The optimization of filters can further increase their brightness, In addition, mNeonGreen appears to be more stable than EGFP and less sensitive to laser induced bleaching. Therefore, mNeonGreen is particularly suitable for confocal microscopy and super-resolution microscopy, especially when studying low-level expression of fusion proteins.
Category: Polyclonal Antibodies
Applications: WB, IF/ICC, ELISA
Cross-reactivity: Species independent
Protein Weight: 30kDa
Observed Molecular Weight: 30kDa
Immunogen: Recombinant protein (or fragment)
Species: Branchiostoma lanceolatum
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:1000 - 1:5000 IF/ICC, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Tagged protein.
NCBI Alias: mNeonGreen
Research Use Only