p53 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A21630-20, A21630-100
Citations, Manuals and MSDS Available upon request.
Background: This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, IF/ICC, IP, ELISA
Cross-reactivity: Human, Mouse
Protein Weight: 43kDa
Observed Molecular Weight: Refer to figures
Immunogen: Synthetic peptide .
Species: Mouse
GeneID: 22059
SWISS: P02340
Alternate Names: bbl; bfy; bhy; p44; p53; Tp53
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 IHC-P, 1:50 - 1:200 IF/ICC, 1:50 - 1:200 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics & Nuclear Signaling, Transcription Factors, DNA Damage & Repair, Cancer, Tumor suppressors, p53 pathway, Signal Transduction, PI3K-Akt Signaling Pathway, ErbB-HER Signaling Pathway, MAPK-JNK Signaling Pathway, MAPK-P38 Signaling Pathway, Cell Biology & Developmental Biology, Apoptosis, Mitochondrial Control of Apoptosis, Cell Cycle, Cell cycle inhibitors, G1/S Checkpoint, G2/M DNA Damage Checkpoint, Endocrine & Metabolism, AMPK Signaling Pathway, Warburg Effect, Neuroscience, Neurodegenerative Diseases.
NCBI Alias: Trp53
Research Use Only