POLR2A Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A11181-20, A11181-100
Citations, Manuals and MSDS Available upon request.
Background: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, IF/ICC, ELISA, ChIP
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 217kDa
Observed Molecular Weight: 250kDa
Immunogen: Synthetic peptide
Species: Human
GeneID: 5430
SWISS: P24928
Alternate Names: RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; NEDHIB; RpIILS; hsRPB1; hRPB220; POLR2A
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 IHC-P, 1:50 - 1:200 IF/ICC, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP, 5μg antibody for 10μg-15μg of Chromatin
Research Areas: Epigenetics Nuclear Signaling, DNA Damage Repair, Protein phosphorylation, Neuroscience, Neurodegenerative Diseases.
NCBI Alias: POLR2A
Research Use Only