Pyruvate carboxylase (PC) Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A6301-20, A6301-100
Citations, Manuals and MSDS Available upon request.
Background: This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.
Category: Polyclonal Antibodies
Applications: WB, IF/ICC, IP, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 130kDa
Observed Molecular Weight: 130kDa
Immunogen: Recombinant protein (or fragment) .
Species: Human
GeneID: 5091
SWISS: P11498
Alternate Names: PCB; Pyruvate carboxylase (PC)
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 IF/ICC, 1:10 - 1:100 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Cancer, Signal Transduction, Endocrine Metabolism, Lipid Metabolism, Carbohydrate metabolism.
NCBI Alias: PC
Research Use Only