Rad21 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A18850-20, A18850-100
Citations, Manuals and MSDS Available upon request.
Background: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, IF/ICC, IP, ELISA, ChIP
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 72kDa
Observed Molecular Weight: 130kDa
Immunogen: Recombinant protein (or fragment) .
Species: Human
GeneID: 5885
SWISS: O60216
Alternate Names: MGS; HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21; Rad21
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 IHC-P, 1:50 - 1:200 IF/ICC, 1:50 - 1:200 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP, 5μg antibody for 10μg-15μg of Chromatin
Research Areas: Epigenetics Nuclear Signaling, DNA Damage Repair, Cancer, Cell Biology Developmental Biology, Apoptosis, Cell Cycle.
NCBI Alias: RAD21
Research Use Only