Raf1 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A0223-20, A0223-100
Citations, Manuals and MSDS Available upon request.
Background: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, IF/ICC, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 73kDa
Observed Molecular Weight: 73kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 5894
SWISS: P04049
Alternate Names: NS5; CRAF; Raf-1; c-Raf; CMD1NN; Raf1
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 IHC-P, 1:50 - 1:200 IF/ICC, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Protein phosphorylation, Cancer, Signal Transduction, G protein signaling, G-Protein-Coupled Receptors Signaling to MAPK Erk, Kinase, Serine threonine kinases, ErbB-HER Signaling Pathway, MAPK-Erk Signaling Pathway, Cell Biology Developmental Biology, Apoptosis, Inhibition of Apoptosis, Growth factors, ESC Pluripotency and Differentiation, Endocrine Metabolism, Mitochondrial metabolism, Mitochondrial markers, Insulin Receptor Signaling Pathway, Immunology Inflammation, B Cell Receptor Signaling Pathway, T Cell Receptor Signaling Pathway, Jak-Stat-IL-6 Receptor Signaling Pathway, Neuroscience.
NCBI Alias: RAF1
Research Use Only