Recombinant Human CCL2/MCP-1 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP01411-10, RP01411-20, RP01411-50, RP01411-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human CCL2/MCP-1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Gln24-Thr99) of human CCL2/MCP-1 (Accession #NP_002973.1) fused with no additional amino acid.
Alternate Names: CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF
SWISS: P13500
GeneID: 6347
Species: Human
Purity: ≥ 90 % as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Monocyte chemoattractant protein 1 (MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection. The presence of MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance.
Bio-Activity: 1.Measured by the ability to inhibit the proliferation of HUVEC(Human Umbilical Vein Endothelial Cells).The ED50 for this effect is 0.53-2.12 ng/mL.|2.Measured by its ability to chemoattract THP-1 cells. The ED50 for this effect is typically 1.01-4.04 ng/mL.
Source: E. coli
Tag: No tag
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT
Endotoxin: < 1 EU/μg of the protein by LAL method.
Research Use Only