Recombinant Human CDH3/P-cadherin Protein
Sizes: 10ug, 20ug, 50ug
Catalogue Numbers: RP01491-10, RP01491-20, RP01491-50
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human CDH3/P-cadherin Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp108-Gly654) of human CDH3/Cadherin-3 (Accession #NP_001784.2) fused with a 6×His tag at the C-terminus.
Alternate Names: CDH3, CDHP, HJMD, PCAD
SWISS: P22223
GeneID: 1001
Species: Human
Purity: ≥ 85 % as determined by SDS-PAGE.
Storage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Cadherin-3 (also known as P-cadherin) is a classical cell-to-cell adhesion molecule with a homeostatic function in several normal tissues. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. In humans, cadherin-3 is only detected in a few organs. However, its overexpression is strongly associated with a poor prognosis in some solid tumors including breast, lung and pancreatic cancers.
Bio-Activity: Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 1×105 cells/well are added to rhP-CAD/Fc Chimera coated plates (10 μg/mL with 100 μL/well), approximately 50-60% will adhere after 1.5 hours at 37° C.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: DWVVAPISVPENGKGPFPQRLNQLKSNKDRDTKIFYSITGPGADSPPEGVFAVEKETGWLLLNKPLDREEIAKYELFGHAVSENGASVEDPMNISIIVTDQNDHKPKFTQDTFRGSVLEGVLPGTSVMQVTATDEDDAIYTYNGVVAYSIHSQEPKDPHDLMFTIHRSTGTISVISSGLDREKVPEYTLTIQATDMDGDGSTTTAVAVVEILDANDNAPMFDPQKYEAHVPENAVGHEVQRLTVTDLDAPNSPAWRATYLIMGGDDGDHFTITTHPESNQGILTTRKGLDFEAKNQHTLYVEVTNEAPFVLKLPTSTATIVVHVEDVNEAPVFVPPSKVVEVQEGIPTGEPVCVYTAEDPDKENQKISYRILRDPAGWLAMDPDSGQVTAVGTLDREDEQFVRNNIYEVMVLAMDNGSPPTTGTGTLLLTLIDVNDHGPVPEPRQITICNQSPVRQVLNITDKDLSPHTSPFQAQLTDDSDIYWTAEVNEEGDTVVLSLKKFLKQDTYDVHLSLSDHGNKEQLTVIRATVCDCHGHVETCPGPWKGG
Endotoxin: < 1 EU/μg of the protein by LAL method.
Research Use Only