Recombinant Human HMGB1-EGFP Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP01737-10, RP01737-20, RP01737-50, RP01737-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human HMGB1-EGFP Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence ((Met1-Glu215)＆ (GGGS) 3＆EGFP) of human HMGB1-EGFP (Accession #NP_002119.1) fused with and a 6×His tag at the C-terminus.
Alternate Names: HMG1; HMG3; HMG-1; SBP-1; HMGB1-EGFP
SWISS: P09429
GeneID: 3146
Species: Human
Purity: ≥ 95% as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2, and HMGB3. HMGB1 is a DNA-binding nuclear protein, released actively following cytokine stimulation as well as passively during cell death. It is the prototypic damage-associated molecular pattern (DAMP) molecule and has been implicated in several inflammatory disorders. As a non-histone nuclear protein, HMGB1 has a dual function. Inside the cell, HMGB1 binds DNA, regulating transcription, and determining chromosomal architecture. Outside the cell, HMGB1 can serve as an alarmin to activate the innate system and mediate a wide range of physiological and pathological responses. Extracellular HMGB1 represents an optimal " necrotic marker" selected by the innate immune system to recognize tissue damage and initiate reparative responses. However, extracellular HMGB1 also acts as a potent pro-inflammatory cytokine that contributes to the pathogenesis of diverse inflammatory and infectious disorders. HMGB1 has been successfully therapeutically targeted in multiple preclinical models of infectious and sterile diseases including arthritis. As shown in studies on patients as well as animal models, HMGB1 can play an important role in the pathogenesis of the rheumatic disease, including rheumatoid arthritis, systemic lupus erythematosus, and polymyositis among others. Besides, enhanced postmyocardial infarction remodeling in type 1 diabetes mellitus was partially mediated by HMGB1 activation.
Source: HEK293 cells
Tag: C-6His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only