Recombinant Human IFN-alpha 4 Protein
Sizes: 20ug, 50ug, 100ug
Catalogue Numbers: RP01914-20, RP01914-50, RP01914-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Recombinant Human IFN-alpha 4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys24-Asp189) of Human IFN-alpha 4(Accession #NP_066546.1) fused with a His tag at the C-terminus.
Alternate Names: Interferon alpha-4, IFN-alpha-4, Interferon alpha-4B, Interferon alpha-76, Interferon alpha-M1, IFNA4
SWISS: P05014
GeneID: 3441
Species: Human
Purity: ≥ 90 % as determined by SDS-PAGE.
Storage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: IFN-alpha 4,Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF-alpha induced signaling. Both the human and mouse genome code for more than a dozen closely related IFN-alpha subtypes and the various IFN-alphas share about 80% sequence homology among them. The mouse IFN-alpha 4 consists of 186 amino acids (aa) including a 24 aa signal peptide and a 162 aa IFN-alpha 4 mature domain. The mature mouse IFN-alpha 4 shares 58% and 76% sequence identity with mature human and rat IFN-alpha 4, respectively. The mouse IFN-alpha 4 reduces the local replication of murine cytomegalovirus in the tibia muscle. mIFN-A4 is a strong activator of Mx gene and was shown to be very effective in reducing splenomegaly.
Bio-Activity: Measured in a cell cytotoxicity assay using TF-1 cells. The ED50 for this effect is 0.26‑1.02 ng/mL, corresponding to a specific activity of 9.80×10^5 ~3.85×10^6 units/mg.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Antigen Sequence: CDLPQTHSLGNRRALILLAQMGRISHFSCLKDRHDFGFPEEEFDGHQFQKAQAISVLHEMIQQTFNLFSTEDSSAAWEQSLLEKFSTELYQQLNDLEACVIQEVGVEETPLMNEDSILAVRKYFQRITLYLTEKKYSPCAWEVVRAEIMRSLSFSTNLQKRLRRKD
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only