Recombinant Human IL-4 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP00995-10, RP00995-20, RP00995-50, RP00995-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human IL-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His25-Ser153) of human IL-4 (Accession #NP_000580.1) fused with a 6×His tag at the C-terminus.
Alternate Names: BCGF-1, BCGF1, BSF-1, BSF1, IL-4, IL4, BCGF1, BSF-1, BSF1, IL-4
SWISS: P05112-1
GeneID: 3565
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE.≥ 95 % as determined by HPLC.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype.
Bio-Activity: Measured in a cell proliferation assay using TF 1 human erythroleukemic cells. The ED50 for this effect is 85-340 pg/mL, corresponding to a specific activity of 2.94 × 10^6 ~1.17× 10^7 units/mg.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
Antigen Sequence: HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only