Recombinant Human Kininogen-1/KNG1 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP01026-10, RP01026-20, RP01026-50, RP01026-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human Kininogen-1/KNG1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln 19 - Ser 427 ) of human Kininogen-1 (Accession #NP_000884) fused with a 6×His tag at the C-terminus.
Alternate Names: BDK, BK, KNG, KNG1, BK, KNG
SWISS: P01042-2
GeneID: 3827
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Kininogen-1 (KNG1) is also known as high molecular weight kininogen, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, Williams-Fitzgerald-Flaujeac factor, which can be cleaved into the following 6 chains:Kininogen-1 heavy chain, T-kinin, Bradykinin, Lysyl-bradykinin, Kininogen-1 light chain, Low molecular weight growth-promoting factor. Kininogen-1 is a secreted protein which contains three cystatin domains. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII. As with many other coagulation proteins, the protein was initially named after the patients in whom deficiency was first observed.Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
Bio-Activity: Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value approximately is 1 nM.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
Antigen Sequence: QESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRMTYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLGMISLMKRPPGFSPFRSSRIGEIKEETTSHLRSCEYKGRPPKAGAEPASEREVS
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only