Recombinant Human PD-1/PDCD1/CD279 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP00067-10, RP00067-20, RP00067-50, RP00067-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human PD-1/PDCD1/CD279 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu25-Thr168) of human PD-1 (Accession #NP_005009.2) fused with a 6×His tag at the C-terminus.
Alternate Names: PDCD1, CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1, programmed cell death 1, PD-1, CD279, PD1, SLEB2, hPD-1, hPD-l, hSLE1, PD-1/CD279
SWISS: Q15116
GeneID: 5133
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE; ≥ 90 % as determined by HPLC.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: This protein is a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure.
Bio-Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human PD-1 at 5 μg/mL (100 μL/well) can bind Recombinant Human PD-L1 with a linear range of 0.5-2 μg/mL.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
Antigen Sequence: LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQT
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only