Recombinant Human TIE2/TEK/CD202b Protein
Sizes: 20ug, 50ug, 100ug
Catalogue Numbers: RP00122-20, RP00122-50, RP00122-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human TIE2/TEK/CD202b Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala23-Lys745) of human Tie2/CD202b/TEK (Accession #NP_000450.2) fused with an Fc, 6×His tag at the C-terminus.
Alternate Names: TEK, CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1, Tie2
SWISS: Q02763
GeneID: 7010
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts.
Bio-Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ANGP2 at 1 μg/mL (100 μL/well) can bind Human Tie2 with a linear range of 3.98-467.9 ng/mL.|2.Measured by its binding ability in a functional ELISA.Immobilized PE anti-human CD202b (Tie2/Tek) Antibody at 1μg/mL (25 μL/well) can bind Human CD202b (Tie2/Tek) with a linear range of 0.46-33.95 ng/mL.
Source: HEK293 cells
Tag: C-hFc&His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
Antigen Sequence: AMDLILINSLPLVSDAETSLTCIASGWRPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGEAIRIRTMKMRQQASFLPATLTMTVDKGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPECNHLCTACMNNGVCHEDTGECICPPGFMGRTCEKACELHTFGRTCKERCSGQEGCKSYVFCLPDPYGCSCATGWKGLQCNEACHPGFYGPDCKLRCSCNNGEMCDRFQGCLCSPGWQGLQCEREGIPRMTPKIVDLPDHIEVNSGKFNPICKASGWPLPTNEEMTLVKPDGTVLHPKDFNHTDHFSVAIFTIHRILPPDSGVWVCSVNTVAGMVEKPFNISVKVLPKPLNAPNVIDTGHNFAVINISSEPYFGDGPIKSKKLLYKPVNHYEAWQHIQVTNEIVTLNYLEPRTEYELCVQLVRRGEGGEGHPGPVRRFTTASIGLPPPRGLNLLPKSQTTLNLTWQPIFPSSEDDFYVEVERRSVQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARVNTKAQGEWSEDLTAWTLSDILPPQPENIKISNITHSSAVISWTILDGYSISSITIRYKVQGKNEDQHVDVKIKNATITQYQLKGLEPETAYQVDIFAENNIGSSNPAFSHELVTLPESQAPADLGGGK
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only