Recombinant Human TNF-alpha Protein
Sizes: 20ug, 50ug, 100ug
Catalogue Numbers: RP00993-20, RP00993-50, RP00993-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human TNF-alpha Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val 77 - Leu 233 ) of human TNF-alpha (Accession #NP_000585.2) fused with a 6×His tag at the C-terminus.
Alternate Names: TNF, DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F, tumor necrosis factor, TNF-α, DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F, TNF alpha
SWISS: P01375
GeneID: 7124
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Tumor necrosis factor alpha (TNF-alpha), also kNown as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. KNockout studies in mice also suggested the neuroprotective function of this cytokine.
Bio-Activity: Recombinant Human TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 0.90-3.60 ng/mL, corresponding to a specific activity of 2.78×10^5 ~1.11×10^6 units/mg.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
Antigen Sequence: VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only