Recombinant Human TNFRSF7/CD27 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP01358-10, RP01358-20, RP01358-50, RP01358-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human TNFRSF7/CD27 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr21-Ile192) of human CD27/TNFRSF7 (Accession #NP_001233.1) fused with a Fc, 6×His tag at the C-terminus.
Alternate Names: CD27, S152, S152. LPFS2, T14, TNFRSF7, Tp55, S152.LPFS2, S152. LPFS2, T14, TNFRSF7, Tp55
SWISS: P26842
GeneID: 939
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.
Bio-Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD27 at 2 μg/mL (100 μL/well) can bind Human CD70 with a linear range of 0.781-0.885 ng/mL.
Source: HEK293 cells
Tag: C-hFc&His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: TPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLSTHWPPQRSLCSSDFIRI
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only