Recombinant Human Triosephosphate isomerase/TPI1 Protein
Sizes: 10ug, 50ug
Catalogue Number: RP02792LQ-10, RP02792LQ-50
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human Triosephosphate isomerase/TPI1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Gln249) of human Triosephosphate isomerase/TPI1 (Accession #NP_000356.1) fused with a 6×His tag at the N-terminus.
Alternate Names: Triosephosphate Isomerase; TIM; Triose-Phosphate Isomerase; TPI1; TPI
SWISS: P60174
GeneID: 7167
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Background: Triose-phosphate isomerase, also named Triose-phosphate isomerase, TPI and TIM, is an enzyme that catalyzes the reversible interconversion of the triose phosphate isomers dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate. TPI has been found in nearly every organism searched for the enzyme, including animals such as mammals and insects as well as in fungi, plants, and bacteria. However, some bacteria that do not perform glycolysis, like ureaplasmas, lack TPI. TPI plays an important role in glycolysis and is essential for efficient energy production. TPI deficiency is an autosomal recessive disorder and the most severe clinical disorder of glycolysis. Triose phosphate isomerase deficiency is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection and characterized by chronic hemolytic anemia.
Source: E. coli
Tag: N-His
Formulation: Supplied as a 0.22 μm filtered solution in 20mM Tris-HCl, 1mM DTT, 10% Glycerol, pH 8.0.
Antigen Sequence: MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ
Endotoxin: < 1 EU/μg of the protein by LAL method.
Research Use Only