Recombinant Human Trk-B/NTRK2 Protein
Sizes: 20ug, 50ug, 100ug
Catalogue Numbers: RP00290-20, RP00290-50, RP00290-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human Trk-B/NTRK2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys32-His430) of human TrkB (Accession #NP_001018074.1) fused with a 6×His tag at the C-terminus.
Alternate Names: NTRK2, GP145-TrkB, TRKB, trk-B, BDNF/NT-3 growth factors receptor, GP145-TrkB, TRKB, trk-B, TrkB, OBHD
SWISS: Q16620
GeneID: 4915
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family and contains two Ig-like C2-type (immunoglobulin-like) domains, two LRR (leucine-rich) repeats, one LRRCT domain, one LRRNT domain, one protein kinase domain.TrkB/NTRK2 tyrosine kinase is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K and PLCgamma signaling pathways.TrkB/NTRK has high affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, longterm potentiation and apoptosis of CNS neurons. As a membrane-bound receptor, TrkB/NTRK binds upon neurotrophin, phosphorylates itself and members of the MAPK pathway. NTRK2 / TrkB involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.
Bio-Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant HumanTrkB, His Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human BDNF with a linear range of 1.95-258 ng/mL.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
Antigen Sequence: CPTSCKCSASRIWCSDPSPGIVAFPRLEPNSVDPENITEIFIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAHKAFLKNSNLQHINFTRNKLTSLSRKHFRHLDLSELILVGNPFTCSCDIMWIKTLQEAKSSPDTQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEEGKSITLSCSVAGDPVPNMYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLIAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPSTDVTDKTGREH
Endotoxin: < 1 EU/μg of the protein by LAL method.
Research Use Only