Recombinant Human Ubiquitin protein
Sizes: 20ug, 50ug, 100ug
Catalogue Numbers: RP01960-20, RP01960-50, RP01960-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human Ubiquitin protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Gly76) of Human Ubiquitin (Accession #NP_001268645.1) fused with a His tag at the N-terminus.
Alternate Names: UBB, Polyubiquitin-B, Cleaved into: Ubiquitin
SWISS: P0CG47
GeneID: 7314
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: UBB is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin. In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes. Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting.
Source: E. coli
Tag: N-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only