Recombinant Human Ubiquitin thioesterase L1/UCHL1 protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP03231-10, RP03231-20, RP03231-50, RP03231-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human Ubiquitin thioesterase L1/UCHL1 protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ala223)of Human Ubiquitin thioesterase L1/UCHL1 (Accession #NP_004172.2) fused with a His tag at the C-terminus.
Alternate Names: UCHL1, Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, EC:3.4.19.12, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1
SWISS: P09936
GeneID: 7345
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.
Source: E. coli
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of 20mM Tris-HCl, 250mM NaCl, 0.05% Tween80, 1mM DTT, pH 8.5.
Antigen Sequence: MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA
Endotoxin: < 1 EU/μg of the protein by LAL method.
Research Use Only