Recombinant Mouse Carbonic Anhydrase IX/CA9 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP01495-10, RP01495-20, RP01495-50, RP01495-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Mouse Carbonic Anhydrase IX/CA9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln32-Asp390) of mouse Carbonic Anhydrase IX/CA9 (Accession #NP_647466.2) fused with a 6×His tag at the C-terminus.
Alternate Names: CAIX, CA9, CA-IX, G250, MN, P54, 58N, pMW1, CA9
SWISS: Q8VHB5
GeneID: 230099
Species: Mouse
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.
Bio-Activity: Measured by its esterase activity. The specific activity is >750 pmol/min/µg, as measured under the described conditions.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: QPQGLSGMQGEPSLGDSSSGEDELGVDVLPSEEDAPEEADPPDGEDPPEVNSEDRMEESLGLEDLSTPEAPEHSQGSHGDEKGGGHSHWSYGGTLLWPQVSPACAGRFQSPVDIRLERTAFCRTLQPLELLGYELQPLPELSLSNNGHTVQLTLPPGLKMALGPGQEYRALQLHLHWGTSDHPGSEHTVNGHRFPAEIHVVHLSTAFSELHEALGRPGGLAVLAAFLQESPEENSAYEQLLSHLEEISEEGSKIEIPGLDVSALLPSDLSRYYRYEGSLTTPPCSQGVIWTVFNETVKLSAKQLHTLSVSLWGPRDSRLQLNFRATQPLNGRTIEASFPAAEDSSPEPVHVNSCFTAGD
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only