Recombinant Mouse NCAM-1/CD56 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP01943-10, RP01943-20, RP01943-50, RP01943-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Mouse NCAM-1/CD56 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu20-Thr711) of Mouse NCAM-1/CD56(Accession #P13595) fused with a Avi&His tag at the C-terminus.
Alternate Names: Ncam1, Ncam, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1, CD56
SWISS: P13595
GeneID: 17967
Species: Mouse
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Neural cell adhesion molecule 1 (NCAM-1; also CD56) is a membrane-bound glycoprotein that plays an important role in nervous system development and function. Mature mouse NCAM-1 consists of a 692 amino acid (aa) extracellular domain (ECD) with five tandem Ig-like domains and two fibronectin type III domains, an 18 aa transmembrane segment, and a 386 aa cytoplasmic domain. Three major splice variants of NCAM-1 are expressed: the 180 kDa full length NCAM-180 isoform, the 140 kDa NCAM-140 isoform which lacks most of the cytoplasmic domain, and the 120 kDa GPI-anchored NCAM-120 isoform that includes the ECD only. Splicing is tissue specific and developmentally regulated. Within the ECD, mouse NCAM-1 shares 94% and 95% aa sequence identity with human and rat NCAM-1, respectively. It is expressed on neurons and glial cells, skeletal muscle, and immune NK cells. NCAM-1 is extensively modified with polysialic acid (PSA) during development, but this addition is decreased in adult tissues. Polysialylation of NCAM-1 is retained in the adult hippocampus where it is important for synaptic plasticity and memory formation. The PSA moiety also participates in the binding of NCAM-1 to heparan sulfate proteoglycans and NCAM-1 mediated migration of olfactory neurons. Proteolytic shedding of NCAM-1 liberates a soluble ECD fragment that can inhibit cortical neurite branching and growth. The NCAM-140 isoform is preferentially expressed on NK cells that robustly secrete cytokines upon activation. Selective up‑regulation of the NCAM-140 isoform in a variety of tumors initiates epithelial-mesenchymal transition (EMT) and promotes tumor cell invasion. Finally, NCAM-1 is known to interact with a number of transmembrane and extracellular molecules. NK cell NCAM-1 binds to T cell FGF R1, co-stimulating IL-2 production by T cells. NCAM-1 also forms a noncovalent membrane complex with GFR alpha 1, 2 and 4, generating a receptor for GDNF, NTN and PSP, respectively. And NCAM-1 is reported to form homophilic trans-interactions, and to interact with L1 CAM in cis, and with HSPGs (agrin and collagen XVIII) in trans. In general, these interactions are involved in cell adhesion, migration, and/or process extension.
Source: HEK293 cells
Tag: C-Avi&His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKTLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEFQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEESWHFKWYDAKEANMEGIVTIMGLKPETRYSVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST
Endotoxin: < 0.01 EU/μg of the protein by LAL method
Research Use Only