Recombinant SARS-CoV-2 Spike RBD Protein
Sizes: 50ug, 100ug
Catalogue Number: RP01258-50, RP01258-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant SARS-CoV-2(2019-nCoV) Spike RBD-His Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of SARS-COV-2(2019-nCoV) Spike RBD-His (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.
Alternate Names: Envelope, SARS-CoV-2 Spike RBD (N501Y), Spike, Spike ECD, Spike RBD, Spike S1, Spike S2, Spike S2 ECD, S1-RBD protein, NCP-CoV RBD Protein, novel coronavirus RBD Protein, 2019-nCoV RBD Protein, S glycoprotein Subunit1 RBD Protein
GeneID: 43740568
Species: SARS-CoV-2
Purity: ≥ 95 % as determined by SDS-PAGE; ≥ 95 % as determined by HPLC.
Storage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Bio-Activity: 1.Measured by its binding ability in a functional ELISA.Immobilized SARS-CoV-2 Spike RBD (Catalog: RP01258) at 2 μg/mL (100 μL/well) can bind Human ACE-2 (Catalog: RP01275) with a linear range of 0.001-2.96 ng/mL.|2.Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 35.3 nM as determined in a SPR assay (Nicoya OpenSPR).|3.Measured by its binding ability in a functional ELISA.Immobilized SARS-CoV-2 Spike RBD (Catalog: RP01258) at 2 μg/mL (100 μL/well) can bind Human ACE-2 (Catalog: RP01275) with a linear range of 0.001-1.69 ng/mL.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4. Contact us for customized product form or formulation.
Antigen Sequence: RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only