RFC4 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A5485-20, A5485-100
Citations, Manuals and MSDS Available upon request.
Background: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
Category: Polyclonal Antibodies
Applications: WB, IF/ICC, IP, ELISA
Cross-reactivity: Human, Rat
Protein Weight: 40kDa
Observed Molecular Weight: 40kDa
Immunogen: Recombinant protein (or fragment) .
Species: Human
GeneID: 5984
SWISS: P35249
Alternate Names: A1; RFC37; RFC4
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 IF/ICC, 1:50 - 1:100 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling.
NCBI Alias: RFC4
Research Use Only