ROCK1 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A0144-20, A0144-100
Citations, Manuals and MSDS Available upon request.
Background: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
Category: Polyclonal Antibodies
Applications: WB, IP, IF-P, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 158kDa
Observed Molecular Weight: 158kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 6093
SWISS: Q13464
Alternate Names: ROCK-I; P160ROCK; ROCK1
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells IF-P, 1:100 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Cancer, Signal Transduction, G protein signaling, Kinase, Serine threonine kinases, Cell Biology Developmental Biology, Apoptosis, Cell Cycle, Centrosome, Cell Adhesion, Cytoskeleton, Microfilaments, Microtubules, Actins, Death Receptor Signaling Pathway, TGF-b-Smad Signaling Pathway.
NCBI Alias: ROCK1
Research Use Only