SARS-CoV-2 Spike Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A20137-20, A20137-100
Citations, Manuals and MSDS Available upon request.
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
Category: Polyclonal Antibodies
Applications: WB, DB, IF/ICC, IP, ELISA
Cross-reactivity: SARS-CoV-2
Protein Weight: 141kDa
Observed Molecular Weight: 110kDa/180kDa
Immunogen: Synthetic peptide
GeneID: 43740568
SWISS: P0DTC2
Alternate Names: spike glycoprotein; SARS-CoV-2 Spike
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 DB, 1:500 - 1:2000 IF/ICC, 1:50 - 1:200 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NCBI Alias: Spike
Research Use Only