Smad4 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A5657-20, A5657-100
Citations, Manuals and MSDS Available upon request.
Background: This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF) -beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE) . The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasing blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, IF/ICC, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 60kDa
Observed Molecular Weight: 63kDa
Immunogen: Recombinant protein (or fragment) .
Species: Human
GeneID: 4089
SWISS: Q13485
Alternate Names: JIP; DPC4; MADH4; MYHRS; Smad4
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 IHC-P, 1:50 - 1:200 IF/ICC, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling, Transcription Factors, Cancer, Tumor suppressors, Signal Transduction, MAPK-JNK Signaling Pathway, Cell Biology Developmental Biology, Apoptosis, Cell Cycle, Cell Cycle Control-G1 S Checkpoint, TGF-b-Smad Signaling Pathway, ESC Pluripotency and Differentiation, Endocrine Metabolism, Stem Cells, Cardiovascular, Hypoxia, Heart, Cardiogenesis, Hypertrophy.
NCBI Alias: SMAD4
Research Use Only