U2AF2/U2AF65 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A1936-20, A1936-100
Citations, Manuals and MSDS Available upon request.
Background: U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
Category: Polyclonal Antibodies
Applications: WB, IF/ICC, IP, ELISA
Cross-reactivity: Human, Mouse
Protein Weight: 54kDa
Observed Molecular Weight: 54kDa
Immunogen: Recombinant protein (or fragment) .
Species: Human
GeneID: 11338
SWISS: P26368
Alternate Names: U2AF65; U2AF2/U2AF65
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 IF/ICC, 1:50 - 1:100 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling, RNA Binding.
NCBI Alias: U2AF2
Research Use Only