14-3-3 zeta (Phospho-T232) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS64514-50, BS64514-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P63104
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB:1:500~1:1000 IHC:1:50~1:200
Background: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: 14-3-3 zeta (Phospho-T232) polyclonal antibody detects endogenous levels of 14-3-3 zeta protein only when phosphorylated at Thr232.
Bioworld Molecular Weight: ~ 28 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of 14-3-3 zeta (Phospho-T232) polyclonal antibody at 1:500 dilution
Lane A: Hela whole cell lysate
Lane B: AGS whole cell lysate
Lane C: The Brain tissue lysate of Rat
Lane D: The Spleen tissue lysate of Rat
Alternative Name: 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; YWHAZ; 14-3-3 protein zeta; 14-3-3 protein delta; 14-3-3 ζ; 14-3-3 δ; 1433Z; KCIP1; KCIP 1
Immunogen: Synthetic phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of Threonine 232.
Conjugate: Unconjugated
Modification: Phosphorylation