20S Proteasome α3 (phospho Ser250) rabbit pAb
Sizes: 50μL, 100μL
Catalogue Numbers: ES6856-50, ES6856-100
Citations, Manuals and MSDS Available upon request.
Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
Alternate Name: PSMA3; HC8; PSC8; Proteasome subunit alpha type-3; Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; Proteasome component C8
Source: Rabbit
Applications: WB; IHC; IF; ELISA
Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Reactivity: Human; Mouse; Rat
Immunogen: The antiserum was produced against synthesized peptide derived from human Proteasome alpha3 around the phosphorylation site of Ser250. AA range:206-255
Storage and Stability: -20°C/1 year
Clonality: Polyclonal
Isotype: IgG
Concentration: 1 mg/ml
Observed Band (KD): 32kD
Human Gene ID: 5684
Human SWISS Prot NO: P25788
Subcellular Location: Cytoplasm. Nucleus. Translocated from the cytoplasm into the nucleus following interaction with AKIRIN2, which bridges the proteasome with the nuclear import receptor IPO9.
Research Use Only