4E-BP1 Polyclonal Antibody
Sizes: 50μL, 100μL
Catalogue Numbers: RA21495-50, RA21495-100
Citations, Manuals and MSDS Available upon request.
Background: EIF4EBP1 encodes one member (eukaryotic translation initiation factor 4E binding protein 1) of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions: -20°C/1 year
Recommended dilutions: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Alternative Names: EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Applications: WB; IHC-p; IF (paraffin section) ; ELISA
Species Cross-Reactivity: Mouse; Rat; Monkey
Protein MW (KDa): 12
Source: Rabbit
Research Use Only