53BP1 Polyclonal Antibody
Sizes: 20μL, 50μL, 100μL
Catalogue Numbers: BT-AP00042-20, BT-AP00042-50, BT-AP00042-100
Citations, Manuals and MSDS Available upon request.
Background: The p53 binding proteins 53BP1 and 53BP2 (Bbp) bind to the central DNA-binding domain of wild type p53, but do not bind mutant p53. The central DNA-binding domain of p53 is required for site-specific DNA binding and is frequently mutated in malignant tumors. Binding of 53BP1 to the L3 loop of p53 and of 53BP2 to the L2 loop of p53 confirms that the loop is dependent on p53 conformation. Site-specific binding also suggests that 53BP1 and 53BP2 are involved in p53-mediated tumor suppression. 53BP1 was isolated from H258 cells and is expressed in Jurkat cells in both the cytoplasm and the nucleus. The N-terminus of 53BP2 is localized to the cytoplasm, while the C-terminus might be localized in the nucleus. 53BP1 promotes cell proliferation by binding to p202, whereas 53BP2 induces cell death by binding to Bcl2 and NFkB p65.
Research Areas: Epigenetics and Nuclear Signaling; Cancer
Target Protein: TP53BP1
Applications: WB, IHC-p, IF, ELISA
Reactivity: Human, Mouse, Rat
Clonality: Polyclonal
Host: Rabbit
Isotype: IgG
Full Product Name: 53BP1 Antibody
Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189
Storage: -20°C for 1 year
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration: 1 mg/ml
Molecular Weight (Da): 213574
UniProt Accession: Human: Q12888; Mouse: P70399
Synonyms: TP53BP1; Tumor suppressor p53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1
GeneID: Human: 7158; Mouse: 27223
Research Use Only