AADAT Polyclonal Antibody
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sizes: 50 µl, 100 µl
Catalogue Numbers: BS72182-50, BS72182-100
Swiss-Prot: Q8N5Z0
Host: Rabbit
AADAT Polyclonal Antibody-BS72182
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF/ICC
All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200
Background: This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 46KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of variousLysates, using AADAT antibody at 1:4040 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunohistochemistry of paraffin-embedded human liver using AADAT Rabbit pAb at dilution of 1:200.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Alternative Name: AADAT; KAT2; KATII; KYAT2
Immunogen: Recombinant fusion protein of human AADAT(NP_001273611.1).
Modification: Unmodified
Conjugate: Unconjugated