Acetyl Lys proteins Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: RA21596-50, RA21596-100
Citations, Manuals and MSDS Available upon request.
Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Store at: -20°C/1 year
Recommended Dilutions: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification Technology:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background: Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis. Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport. The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases, and HDACs have become promising targets for anti-cancer drugs currently in development.
Applications: WB, IHC-p, IF/ICC, ELISA
Species Cross-Reactivity: Human, Mouse, Rat, Monkey
Molecular Weight: 20,40,80,175
Protein Molecular Weight (kDa): 20,40,80,175
Source: Rabbit
Immunogen: Synthesized acetyl-peptide derived from human acetylation Lys proteins.
Category: Polyclonal
Research Use Only