Actin Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BZ16045-50, BZ16045-100
Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Swiss-Prot: P60709/P63261
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, ELISA
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000
Background: beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 42 kDa; Observed MW: 45 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Actin in various lysates using Actin antibody., Western blot analysis of Actin in HeLa lysates using Actin antibody., Western blot analysis of Actin in various lysates using Actin antibody., Western blot analysis of Actin in various lysates using Actin antibody.
Alternative Name: ACTB; Actin; cytoplasmic 1; Beta-actin; POTEKP; ACTBL3; FKSG30; Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; ACTG1; ACTB; ACTG; Actin; cytoplasmic 2; Gamma-actin
Immunogen: The antiserum was produced against synthesized peptide derived from human Actin. AA range:326-375
Conjugate: Unconjugated
Modification: Unmodified