AK6 (G35) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS2171-50, BS2171-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9Y3D8
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IP,
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:10~1:100
Background: TAF9(AK6) functions as a component of the DNA-binding general transcription factor complex TFIID and the regulatory transcription complex SAGA. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3 and SPT8) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3, and TRA1), and chromatin modification through histone acetylation (GCN5).
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: AK6 (G35) polyclonal antibody detects endogenous levels of AK6 protein.
Bioworld Molecular Weight: ~ 20 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of AK6 (G35) polyclonal antibody at 1:1000 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: The Lung tissue lysate of Rat (30ug)
Lane 3: The Lung tissue lysate of Mouse (30ug), Immunoprecipitation of HEK293T cell lysate using AK6 (G35) polyclonal antibody (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using ATP5H (D146) #BS2171. “↑”（supernatant）;“↓(deposition）
Alternative Name: Adenylate kinase isoenzyme 6; AK6; Adrenal gland protein AD-004; Coilin-interacting nuclear ATPase protein; hCINAP; Dual activity adenylate kinase/ATPase; AK/ATPase; CINAP; AD-004; CGI-137
Immunogen: Synthetic peptide, corresponding to the N-terminual of Human AK6.
Conjugate: Unconjugated
Modification: Unmodified