AKR1C3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS6774-50, BS6774-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P42330
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, IF/ICC, IP,
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:100
Background: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 37KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of various lysates, using AKR1C3 antibody at 1:1620 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s., Immunofluorescence analysis of NIH/3T3 cells using AKR1C3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 200ug extracts of K-562 cells, using 3 ug AKR1C3 antibody. Western blot was performed from the immunoprecipitate using AKR1C3 antibody at a dilution of 1:1000.
Alternative Name: AKR1C3; DD3; DDX; HA1753; HAKRB; HAKRe; HSD17B5; PGFS; hluPGFS
Immunogen: Recombinant fusion protein of human AKR1C3(NP_003730.4).
Conjugate: Unconjugated
Modification: Unmodified