AKT Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB10510-50, MB10510-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P31749/P31751/Q9Y243
Host: Rabbit
Reactivity: Human, Mouse, Rat, Hamster
Applications: WB, IHC-F, IHC-P, ICC/IF, IP,
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 56 kDa; Observed MW: 56 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of AKT in 3T3, mouse lung lysates using AKT antibody., Western blot analysis of AKT1/2/3 in Jurkat, rat Brain, C6, CHO-K1, Hela lysates using AKT antibody., Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AKT1/2/3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Alternative Name: AKT1
Immunogen: Recombinant protein of human AKT1/2/3
Conjugate: Unconjugated
Modification: Unmodified