ALAD Polyclonal Antibody
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sizes: 50µl, 100µl
Catalogue Numbers: BS74847-50, BS74847-100
Swiss-Prot: P13716
Host: Rabbit
ALAD Polyclonal Antibody-BS74847
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Reactivity: Human, Mouse
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Unmodification
Bioworld Molecular Weight: 36kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using ALAD antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of HeLa cells using ALAD antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Alternative Name: ALAD; ALADH; PBGS
Immunogen: Recombinant fusion protein of human ALAD(NP_000022.3).
Modification: Unmodified
Conjugate: Unconjugated