ALPP Monoclonal Antibody
Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Catalogue Numbers: MB66638-50, MB66638-100
Sizes: 50µl, 100µl
Swiss-Prot: P05187
Host: Mouse
Reactivity: Human
Applications: WB, IHC
Background: Mammalian alkaline phosphatases (APs) are highly conserved zinc-containing allosteric enzymes that are able to hydrolyze and transphosphorylate a wide range of compounds. There are four known human alkaline phosphatase isozymes: TNAP (tissue-nonspecific; bone/liver/kidney), ALPP (placental), ALPP2 (germ cell), and ALPI (intestinal). Placental alkaline phosphatase (ALPP) is bound to the plasma membrane via a glycosyl-phosphatidylinositol (GPI) anchor. It is expressed primarily in the placenta and may be involved in transplacental IgG transport. ALPP has been found to be overexpressed on the surface of several different types of solid tumor cells and elevated serum concentrations of ALPP and ALPP-like enzymes has been found to be associated with ovarian, cervical, and testicular cancer.
Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Purification and Purity: The antibody was purified by immunogen affinity chromatography.
Specificity: Recognizes endogenous levels of ALPP protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of ALPP expression in HepG2 (A), A431 (B), MCF7 (C) whole cell lysates., Immunohistochemical analysis of ALPP staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.141). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Bioworld Molecular Weight: ~ 70 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: PLAP; Alkaline phosphatase placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1
Immunogen: Purified recombinant fragment of human ALPP expressed in E. Coli.
Conjugate: Unconjugated
Modification: Unmodified