ATG7 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS80501-50, BS80501-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O95352
Host: Rabbit
Reactivity: Human
Applications: IHC
All Applications: IHC,1:50 - 1:100
Background: This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Immunogen: Recombinant fusion protein of human ATG7(NP_001129503.2).
Conjugate: Unconjugated
Modification: Unmodified
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemistry of paraffin-embedded human breast cancer using ATG7 antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.