ATGL/PNPLA2 Polyclonal Antibody
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sizes: 50 µl, 100 µl
Catalogue Numbers: BS7989-50, BS7989-100
Swiss-Prot: Q96AD5
Host: Rabbit
ATGL/PNPLA2 Polyclonal Antibody-BS7989
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 55kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using ATGL/PNPLA2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s., Immunofluorescence analysis of U2OS cells using ATGL/PNPLA2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Alternative Name: PNPLA2; 1110001C14Rik; ATGL; FP17548; PEDF-R; TTS-2.2; TTS2; iPLA2zeta
Immunogen: Recombinant fusion protein of human PNPLA2(NP_065109.1).
Modification: Unmodified
Conjugate: Unconjugated