ATP8B3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS77517-50, BS77517-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O60423
Host: Rabbit
Reactivity: Human
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of aminophospholipid-transporting ATPases. The aminophospholipid translocases transport phosphatidylserine and phosphatidylethanolamine from one side of a bilayer to the other. This gene encodes member 3 of phospholipid-transporting ATPase 8B; other members of this protein family are located on chromosomes 1, 15 and 18. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of HCT116 cells, using ATP8B3 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Note: For research use only, not for use in diagnostic procedure.
Bioworld Molecular Weight: 147kDa
Alternative Name: ATPIK; ATP8B3
Immunogen: Recombinant fusion protein of human ATP8B3(NP_620168.1).
Conjugate: Unconjugated
Modification: Unmodified