FEN-1 Rabbit pAb
Sizes: 20µl, 100µl
Catalogue Numbers: A1175-20, A1175-100
Citations, Manuals and SDS Available upon request.
Background: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Synonyms: MF1; RAD2; FEN-1
GeneID: 2237
Swiss Prot: P39748
Tested Applications: ELISA, WB, IHC-P, IF/ICC, IP
Reactivity: Human, Mouse
Calculated Molecular Weight: 43kDa
Observed Molecular Weight: 48kDa
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-380 of human FEN-1 (NP_004102.1).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:2000 | IHC-P: 1:50 - 1:200 | IF/ICC: 1:10 - 1:100 | IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Research Areas: Epigenetics Nuclear Signaling, DNA Damage Repair
Modifications: Unmodified
Conjugate: Unconjugated
Positive Samples: NIH/3T3, HeLa, Jurkat, Y79, Mouse spleen, Mouse testis
Cellular Location: Mitochondrion, Nucleus, Nucleolus, Nucleoplasm,
Keywords: 2237, sc-8794, sc-8795, sc-13051, FEN1, FEN-1, MF1, RAD2, flap structure-specific endonuclease 1, flap endonuclease 1, DNase IV, maturation factor-1, P39748, Flap Structure-Specific Endonuclease 1, Maturation Factor-1, Flap Endonuclease 1, Maturation Factor 1, EC 3.1.-.-, HFEN-1, FEN 1, sc8794, sc 8794, sc8795, sc 8795, sc13051, sc 13051, Flap endonuclease 1, flap structure endonuclease 1, hFEN 1, Maturation factor 1
Symbol: FEN1
Category: Polyclonal Antibodies
Research Use Only