(KO Validated) HIF1AN Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-68371-60, E-AB-68371-120, E-AB-68371-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: HIF1AN
Target Synonym: HIF1AN; FIH1
Research Areas: Cancer, Epigenetics and Nuclear Signaling
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IF
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q9NWT6
Background: Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD. Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
Concentration: 1 mg/mL
Immunogen: Recombinant fusion protein of human HIF1AN
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000, IF 1:50-1:200
Calculated MW: 40kDa
Observed MW: 40kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only