(KO Validated) ROCK1 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-62198-60, E-AB-62198-120, E-AB-62198-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: ROCK1
Target Synonym: ROCK1; P160ROCK; ROCK-I
Research Areas: Cancer, Cell Biology, Signal Transduction
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IHC
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q13464
Background: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human ROCK1 (NP_005397.1).
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000 IHC 1:50-1:100
Calculated MW: 158kDa
Observed MW: 158kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only