IRP-1 Polyclonal Antibody
Sizes: 20μL, 50μL, 100μL
Catalogue Numbers: BT-AP04640-20, BT-AP04640-50, BT-AP04640-100
Citations, Manuals and MSDS Available upon request.
Background: The protein encoded by ACO1 is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The protein encoded by ACO1 has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants.
Research Areas: Cardiovascular
Target Protein: ACO1
Applications: IHC-p, ELISA
Reactivity: Human, Mouse, Rat
Clonality: Polyclonal
Host: Rabbit
Isotype: IgG
Full Product Name: IRP-1 Antibody
Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189
Storage: -20°C for 1 year
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration: 1 mg/ml
Molecular Weight (Da): 98399
UniProt Accession: Human: P21399; Mouse: P28271; Rat: Q63270
Synonyms: ACO1; IREB1; Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1
GeneID: Human: 48; Mouse: 11428; Rat: 50655
Research Use Only