LATS1/2 Rabbit pAb
Sizes: 50μL, 100μL
Catalogue Numbers: ES8905-50, ES8905-100
Citations, Manuals and MSDS Available upon request.
Background: The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatmen
Alternate Name: Serine/threonine-protein kinase LATS1 (EC 2.7.11.1) (Large tumor suppressor homolog 1) (WARTS protein kinase) (h-warts)
Source: Rabbit
Applications: WB; ELISA; IHC
Dilution: WB 1:500-2000; IHC-p 1:50-300; ELISA 2000-20000
Reactivity: Human; Mouse; Rat
Immunogen: Synthesized peptide derived from human LATS1/2 AA range: 1050-1130
Storage and Stability: -20°C/1 year
Clonality: Polyclonal
Isotype: IgG
Concentration: 1 mg/ml
Observed Band (KD): 140kD
Human Gene ID: 9113
Human SWISS Prot NO: O95835
Subcellular Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus including spindle pole bodies, mitotic spindle, and midbody, during mitosis.
Research Use Only